BPC-157 and TB-500 blend: what one draw actually delivers
A 1:1 BPC-157 and TB-500 vial cannot deliver both at the amounts the literature describes for each on its own. Work out what your draw really contains, in both compounds at once.
The most common pre-mixed peptide vial in circulation holds BPC-157 and TB-500 together, usually five milligrams of each at the low end and ten of each further up. It is widely called the Wolverine blend, and the reason people buy it is obvious enough: two repair peptides, one reconstitution, one injection.
The arithmetic is where it gets interesting, and it is not the arithmetic most people expect. Both compounds are dissolved in the same water, so every draw takes them in whatever ratio is in the vial. You cannot dose one without dosing the other. That would be unremarkable if the two were dosed alike. They are not — and the gap is large enough to be the main thing worth knowing before you mix a vial.
volume to draw (ml) = dose wanted (mcg) ÷ that concentration
what the draw delivers of any other = that other (mg) × 1000 × volume ÷ water
units on a U-100 syringe = volume to draw (ml) × 100
Only the last line is specific to a blend, and it is the one people skip. A draw is a fraction of the vial, so it takes the same fraction of every component. Draw a fifth of the vial and you get a fifth of each thing in it.
The ratio problem, in numbers
Each row below anchors on one component at the low end of what the literature and community practice describe for that compound on its own, then shows what the same draw delivers of everything else in the vial. Computed for the reference vial — 5 mg BPC-157, 5 mg TB-500 in 2 ml of bacteriostatic water — and the calculator above will redo it for yours.
| If you dose for | Draw | BPC-157 | TB-500 |
|---|---|---|---|
| BPC-157 at 200 mcg | 0.08 ml (8 u) | 200 mcg | 200 mcg (10% of its own low end) |
| TB-500 at 2 mg | 0.8 ml (80 u) | 2 mg (10× its own low end) | 2 mg |
The amounts each row is anchored to, and where they come from:
- BPC-157 — 200-250mcg/day, Once daily SubQ near injury or orally (Starting, from this site's compound reference)
- TB-500 — 2-2.5mg twice weekly, SubQ or IM (Loading, from this site's compound reference)
Off-label or community practice None of these compounds is approved for human use, and the amounts above are what is described in the literature and in community practice rather than established doses. They are here as the yardstick the blend is being measured against, not as a recommendation. What matters on this page is the gap between the columns: it is a property of the vial's ratio, and no injection technique changes it.
Why this blend is put together the way it is
BPC-157 is a synthetic pentadecapeptide with a modelled half-life of about four hours — an estimate, from limited data — which is why the schedules in circulation are daily or twice daily. TB-500 is a synthetic fragment of thymosin beta-4 with a modelled half-life of about two days, also an estimate, and its schedules are weekly or twice weekly.
So the two compounds disagree about frequency as well as amount. A blend injected daily to suit the first gives seven TB-500 injections a week; injected weekly to suit the second, it gives one BPC-157 injection a week. There is no schedule that is the usual schedule for both, and the vial cannot separate them. Neither compound is approved for human use anywhere, and none of this is a recommendation to run either — it is what the ratio does once the vial is mixed.
What is in the reference vial
- Components
- BPC-157 · TB-500
- Also called
- Wolverine blend, Wolverine stack, BPC/TB blend
- Reference vial
- 5 mg BPC-157 + 5 mg TB-500 in 2 ml bacteriostatic water
- Regulatory status
- BPC-157: Research compound — no FDA approval. Not for human use label.
TB-500: Research compound — no FDA approval. Not for human use label. - Before mixing
- Sealed powder tolerates room temperature in transit, but keep it refrigerated at 2–8 °C (36–46 °F) for anything beyond a few weeks, or frozen for months. Keep it dark — the carton it shipped in is doing a job.
- After mixing
- Once reconstituted with bacteriostatic water: refrigerate at 2–8 °C and use within about 28 days. The benzyl alcohol in bacteriostatic water is what buys that window — plain sterile water has no preservative, so a vial mixed with it should be treated as one sitting only. Kits are usually ten vials: only the one you mixed is on that 28-day clock — the sealed ones keep their own, much longer shelf life, so store them as powder, not as solution.
- What ruins it
- Do not freeze a reconstituted vial. Repeated freeze–thaw is one of the more reliable ways to degrade a peptide.
- Handling caveat
- General handling practice for this formulation. Your supplier’s own insert or COA overrides anything here.
Questions people actually ask
Is this the "Wolverine blend"?
Yes — "Wolverine blend" and "Wolverine stack" are the names this BPC-157 and TB-500 combination circulates under in peptide communities. It is a nickname rather than a product: there is no standard formulation behind it, and two vials sold under the name can hold different amounts and different ratios. That is exactly why this page asks you to enter what is on your own label rather than assuming a recipe.
Is a blend cheaper or more convenient than separate vials?
Cheaper per milligram, often. More convenient, only if the ratio happens to suit you. Separate vials cost more and take two draws, but they let you set each compound's amount and schedule independently, which a fixed-ratio vial cannot. That trade — convenience against control — is the real decision, and the numbers above are what you are trading away.
Can I just inject more to get enough TB-500?
You can draw more, and the calculator will tell you exactly what that costs you in BPC-157, which climbs in lockstep. Whether the resulting amount of either compound is appropriate is not an arithmetic question and this page will not answer it. There is no human trial data establishing a safe amount for either compound, and that is the honest state of the evidence. Take it to a clinician who can look at your whole picture.
Can I separate the components once the vial is mixed?
No. They are dissolved in the same water and go into the syringe together, in whatever ratio is in the vial. The only way to set each compound's amount independently is separate vials.
How do I know what is actually in my vial?
You do not, beyond what the label claims. None of these compounds is sold as an approved medicine, so nothing on the label has been verified by a regulator, and a blend adds a second unknown on top of the first: the ratio. The calculator is exact about the numbers you give it, and those numbers come from a label nobody checked. That is worth holding in mind before treating any output here as precise.
What about storage once it is reconstituted?
Mixed with bacteriostatic water, refrigerated at 2–8 °C and used within about 28 days is the rule the app applies to reconstituted peptides, and it applies to a blend as a whole rather than to each component. General handling practice for this formulation. Your supplier’s own insert or COA overrides anything here.
Does the calculator know these are research compounds?
It does arithmetic, and nothing else. None of the compounds in this vial is approved for human use, and a calculator that tells you a draw is 40 units is not telling you the draw is a good idea. Anything you are considering injecting belongs in a conversation with a doctor who can see your whole picture.
A blend is one vial with several clocks running in it. The app keeps the vial, the concentration and every draw you take from it, so the split only has to be worked out once.
Save this draw to your logThis calculator does the arithmetic you typed and nothing else. It does not know what is actually in your vial, whether the label is accurate, or anything about you. Confirm the vial strength and the diluent volume on your own label before you draw, and take dosing decisions to a qualified provider.